Mechanisms of biochemical reactions within crowded environments
Dissertation, Universität Potsdam, 2016
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| Sprache: | eng |
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Potsdam
2016
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Tag hinzufügen | Zusammenfassung: | Dissertation, Universität Potsdam, 2016 The cell interior is a highly packed environment in which biological macromolecules evolve and function. This crowded media has effects in many biological processes such as protein-protein binding, gene regulation, and protein folding. Thus, biochemical reactions that take place in such crowded conditions differ from diluted test tube conditions, and a considerable effort has been invested in order to understand such differences.In this work, we combine different computationally tools to disentangle the effects of molecular crowding on biochemical processes. First, we propose a lattice model to study the implications of molecular crowding on enzymatic reactions. We provide a detailed picture of how crowding affects binding and unbinding events and how the separate effects of crowding on binding equilibrium act together. Then, we implement a lattice model to study the effects of molecular crowding on facilitated diffusion. We find that obstacles on the DNA impair facilitated diffusion. However, the extent of this effect depends on ho… |
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| Beschreibung: | kumulative Dissertation |
| Beschreibung: | vii, 112 Seiten Illustrationen, Diagramme |