Electric field-assisted immobilization and alignment of biomolecules
Dissertation, Universität Potsdam, 2016
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| Sprache: | eng |
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Potsdam
2016
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Tag hinzufügen | Zusammenfassung: | Dissertation, Universität Potsdam, 2016 In this dissertation, an electric field-assisted method was developed and applied to achieve immobilization and alignment of biomolecules on metal electrodes in a simple one-step experiment. Neither modifications of the biomolecule nor of the electrodes were needed. The two major electrokinetic effects that lead to molecule motion in the chosen electrode configurations used were identified as dielectrophoresis and AC electroosmotic flow. To minimize AC electroosmotic flow, a new 3D electrode configuration was designed. Thus, the influence of experimental parameters on the dielectrophoretic force and the associated molecule movement could be studied. Permanent immobilization of proteins was examined and quantified absolutely using an atomic force microscope. By measuring the volumes of the immobilized protein deposits, a maximal number of proteins contained therein was calculated. This was possible since the proteins adhered to the tungsten electrodes even after switching off the electric field. The permanent immobilization of function… |
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| Beschreibung: | IX, 120 Seiten Illustrationen, Diagramme |