Laboratory methods in enzymology protein Part C
Section I. Protein protocols/protein precipitation. TCA precipitation / Laura Koontz -- Section II. Protein protocols/protein pull-down methods ; Coimmunoprecipitation of proteins from yeast / Erica Gerace and Danesh Moazed ; Coupling antibody to cyanogen bromide-activated Sepharose / Jennifer M. Ka...
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| Format: | UnknownFormat |
| Sprache: | eng |
| Veröffentlicht: |
Amsterdam u.a.
Elsevier, Academic Press
2014
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| Ausgabe: | First edition |
| Schriftenreihe: | Methods in enzymology
541 |
| Schlagworte: | |
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Tag hinzufügen | Zusammenfassung: | Section I. Protein protocols/protein precipitation. TCA precipitation / Laura Koontz -- Section II. Protein protocols/protein pull-down methods ; Coimmunoprecipitation of proteins from yeast / Erica Gerace and Danesh Moazed ; Coupling antibody to cyanogen bromide-activated Sepharose / Jennifer M. Kavran and Daniel J. Leahy ; Analysis of protein-protein interactions by coimmunoprecipitation / Peter van der Geer -- Section III. Protein protocols/protein purification. Use and application of hydrophobic interaction chromatography for protein purification / Justin T. McCue ; Hydroxyapatite chromatography : purification strategies for recombinant proteins / Larry J. Cummings ; Salting out of proteins using ammonium sulfate precipitation / Krisna C. Duong-Ly and Sandra B. Gabelli ; Using ion exchange chromatography to purify a recombinantly expressed protein / Krisna C. Duong-Ly and Sandra B. Gabelli ; Gel filtration chromatography (size exclusion chromatography) of proteins / Krisna C. Duong-Ly, and Sandra B. Gabelli -- Section IV. Protein protocols/purification of membrane proteins. Expression and purification of membrane proteins / Jan Kubicek, Helena Block, Barbara Maertens, Anne Spriestersbach, and Jörg Labahn ; Explanatory chapter : choosing the right detergent / Dirk Linke -- Section V. Protein protocols/SDS PAGE. One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) / Julie L. Brunelle and Rachel Green ; Coomassie blue staining / Julie L. Brunelle and Rachel Green ; Silver staining of SDS-polyacrylamide Gel / Jennifer M. Kavran and Daniel J. Leahy ; Section VI. Protein protocols/standard in vitro assays for protein-nucleic acid interactions. Standard in vitro assays for protein-nucleic acid interactions : Gel shift assays for RNA and DNA binding / Sarah F. Mitchell and Jon R. Lorsch ; Protein filter binding / Sarah Kolitz and Jon R. Lorsch ; Section VII. Protein protocols/troubleshooting protein expression. Explanatory chapter : troubleshooting recombinant protein expression : general / Krisna C. Duong-Ly and Sandra B. Gabelli ; Explanatory chapter : troubleshooting protein expression : what to do when the protein is not soluble / Krisna C. Duong-Ly and Sandra B. Gabelli ; Section VIII. Protein protocols/Western blotting. Western blotting using chemiluminescent substrates / Alice Alegria-Schaffer. |
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| Beschreibung: | Literaturangaben |
| Beschreibung: | xvii, 272 Seiten graph. Darst. |
| ISBN: | 9780124201194 978-0-12-420119-4 |