Making love and war jasmonate signaling in herbivore defense and flower development in Nicotiana attenuata
Jena, Univ., Diss., 2014
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| Sprache: | eng |
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Jena
2014
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Tag hinzufügen | Zusammenfassung: | Jena, Univ., Diss., 2014 The goal of my work presented in this thesis was to investigate the regulatory role of JAs-homeostasis during the herbivore induced defense activation and floral development in the wild tobacco Nicotiana attenuata. Biosynthetic pathways are most commonly investigated by silencing or overexpression of genes whose products catalyze individual steps within the pathway. However, the complete interruption of a pathway per se prevents the full elucidation of the multiple control mechanisms enabling plants to constantly adjust the output of their signaling pathways. To evade aforementioned limitations, we developed a new approach by ectopically expressing in N. attenuata a gene coding for a methyltransferase that specifically catalyzes the methylation of JA to methyljasmonate (MeJA). Using molecular and chemical analytical methods, I demonstrated the efficiency of this enzymatically redirected JA-flux. The examination of JAs-accumulation patterns upon herbivory in locally treated and untreated systemic leaves revealed tissue-specific consequences of creating a metabolic sink in the JAs-cascade. Furthermore, I show that the metabolic rerouting of JA-flux towards MeJA formation caused no effect on the direct or indirect transcriptional regulation of JA-biosynthesi |
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| Beschreibung: | II, 214 S. Ill., graph. Darst. 29,5 cm |