Anaerobic O-demethylation in acetobacterium dehalogenans interaction mediated by O-demethylase components
Dissertation, Friedrich-Schiller-Universität Jena, 2013
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| Sprache: | eng |
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Jena
2013
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Tag hinzufügen | Zusammenfassung: | Dissertation, Friedrich-Schiller-Universität Jena, 2013 In the work presented here, the interactions of the O-demethylase components were studied using two-hybrid assays, gel shift experiments and far-Western blot analyses. Emphasis was laid on studies on the interaction of AE and CP. With the exception of the yeast two-hybrid assay (Y2H), an interaction between AE and CP was always observed. In gel shift experiments, the presence of the corrinoid cofactor was a prerequisite for the interaction of the two components. On native PAGE, AE appeared as dimer. The Y2H experiments pointed to an involvement of the N-terminal fragment of AE (AE 1-133) in the oligomerization of the protein. Interaction studies in the presence of the other O-demethylase components revealed interactions of CP and the two MTs which is in accordance to the proposed reaction mechanism of the O-demethylases. ... |
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| Beschreibung: | IV, 85 Blätter Diagramme 29 cm |