Calcium techniques a laboratory manual
FluorescenceLuminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.
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| Weitere Verfasser: | , , , |
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| Format: | UnknownFormat |
| Sprache: | eng |
| Veröffentlicht: |
Cold Spring Harbor, NY
Cold Spring Harbor Laboratory Press
2014
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| Schlagworte: |
Calcium
> physiology
> Calcium Channels
> Calcium Signaling
> Fluorometry
> methods
> œaCalcium ChannelsœvLaboratory Manuals
> œaCalcium SignalingœvLaboratory Manuals
> œaCalciumœxphysiologyœvLaboratory Manuals
> œaFluorometryœxmethodsœvLaboratory Manuals
> Calciumkanal
> Calciumion
> Fluoreszenzmikroskopie
> Calciumfreisetzung
> Patch-Clamp-Methode
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Tag hinzufügen | Zusammenfassung: | FluorescenceLuminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics. "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"-- |
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| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XVI, 592 S. Ill., graph. Darst. |
| ISBN: | 9781621820789 978-1-62182-078-9 9781936113583 978-1-936113-58-3 |