Cell imaging techniques methods and protocols

Molecular beacons: fluorescent probes for detection of endogenous mRNAs in living cells -- Second-harmonic imaging of collagen -- Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy -- Multifluorescence labeling techniques and confocal laser scanning mi...

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Bibliographische Detailangaben
Weitere Verfasser: Taatjes, Douglas J. (HerausgeberIn), Mossman, Brokke T. (HerausgeberIn)
Format: UnknownFormat
Sprache:eng
Veröffentlicht: Totowa, NJ Humana Press 2010
Schriftenreihe:Methods in molecular biology 319
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Zusammenfassung:Molecular beacons: fluorescent probes for detection of endogenous mRNAs in living cells -- Second-harmonic imaging of collagen -- Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy -- Multifluorescence labeling techniques and confocal laser scanning microscopy on lung tissue -- Evaluation of confocal microscopy system performance -- Quantitative analysis of atherosclerotic lesion composition in mice -- Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases -- Laser scanning cytometry: principles and applications -- Near-clinical applications of laser scanning cytometry -- Laser capture microdissection -- Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse transriptase-polymerase chain reaction -- New approaches to fluorescence in situ hybridization -- Microarray image scanning -- Near-field scanning optical microscopy in cell biology and cytogenetics -- Porosome: the fusion pore revealed by multiple imaging modalities -- Secretory vesicle swelling by atomic force microscopy -- Imaging and probing cell mechanical properties with the atomic force microscopy -- Reflection contrast microscopy: the bridge between light and electron microscopy -- Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition -- Three-dimensional reconstruction of single particles in electron microscopy: image processing -- A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing
Beschreibung:Literaturangaben
Beschreibung:XIV, 490 Seiten
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ISBN:9781617373947
978-1-61737-394-7