Quantification of low-energy electron induced single and double strand breaks in well-defined DNA sequences using DNA origami nanostructures
Dissertation, Universität Potsdam, 2021
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| Sprache: | eng |
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Potsdam
2021
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Tag hinzufügen | Zusammenfassung: | Dissertation, Universität Potsdam, 2021 Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors leading to cell death and reduction of the tumor tissue. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEE) with the most probable energy around 10 eV through ionization of water molecules in the cells. A simulation of the dose distribution in the patient is required to optimize the irradiation modality in cancer radiation therapy, which must be based on the fundamental physical processes of high-energy radiation with the tissue. In the present work the accurate quantification of DNA radiation damage in the form of absolute cross sections for LEE-induced DNA strand breaks (SBs) between 5 and 20 eV is done by using the DNA origami technique. This method is based on the analysis of well-defined DNA target sequences attached to DNA origami triangles with atomic force microscopy (AFM) on the single molecule level. The present work focuses on poly-adenine sequences (5'-d(A4), 5'-d(A8), ... |
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| Beschreibung: | 111 Seiten Illustrationen, Diagramme |