Etablierung eines robusten DNA-Microarray-gestützten Verfahrens zur nicht gerichteten Virusdetektion mit optionaler Kopplung an eine High-Throughput-Sequencing-Technologie
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2020
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Greifswald
29.11.2019
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Tag hinzufügen | Zusammenfassung: | Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2020 Detection, Mikroarray, Virus, DNA-Microarray, High-Throughput-Sequencing-Technologie, Virusdetektion Lipoproteins of Staphylococcus aureus represent a major class of surface proteins, which are anchored to the outer leaflet of the cell membrane. Although they play a key role in the immune response and virulence, the majority of lipoproteins in this organism is still of unknown function. The aim of our study was to investigate the function of so far poorly or uncharacterized lipoproteins in S. aureus strain Newman. To this end, an integrated bioinformatical approach was applied to define the pan-lipoproteome of 123 completely sequenced S. aureus strains. In total, this analysis predicted 192 different potential lipoproteins, with a core lipoproteome of 39 and a variable lipoproteome of 153 lipoproteins. Out of those 192 lipoproteins, 141 are so far functionally uncharacterized. Primarily focusing on members of the core-lipoproteome with unknown or poorly characterized function, 24 lipoproteins or co-encoded neighbor proteins were selected for further characterization. Of those 24 proteins, 20 S. aureus markerless deletion mutants were constructed (S. aureus delta l01 - delta l20) and screened for an altered growth behavior under various conditions. Here, three mutants showed a temperature-sensitive phenotype, two mutants formed aggregates in the TSB of the manufacturer Merck (TSBMerck), and four mutants showed reduced growth under osmotic stress with 8% NaCl. An altered aggregation behavior was observed for four mutants in the presence of Triton X-100 and for eleven mutants ... |
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| Beschreibung: | Literaturverzeichnis: Seite 119-135 |
| Beschreibung: | 137 Seiten Illustrationen (teilweise farbig), Diagramme (teilweise farbig) |