DNA origami structures as versatile platforms for nanophotonics
Dissertation, Universität Potsdam, 2018
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| Sprache: | eng |
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Potsdam
2018
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Tag hinzufügen | Zusammenfassung: | Dissertation, Universität Potsdam, 2018 Nanophotonics is the field of science and engineering aimed at studying the light-matter interactions on the nanoscale. One of the key aspects in studying such optics at the nanoscale is the ability to assemble the material components in a spatially controlled manner. In this work, DNA origami nanostructures were used to self-assemble dye molecules and DNA coated plasmonic nanoparticles. Optical properties of dye nanoarrays, where the dyes were arranged at distances where they can interact by Förster resonance energy transfer (FRET), were systematically studied according to the size and arrangement of the dyes using fluorescein (FAM) as the donor and cyanine 3 (Cy 3) as the acceptor. The optimized design, based on steady-state and time-resolved fluorometry, was utilized in developing a ratiometric pH sensor with pH-inert coumarin 343 (C343) as the donor and pH-sensitive FAM as the acceptor. ... |
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| Beschreibung: | 125 Seiten Illustrationen, Diagramme |