Analytical ultracentrifugation techniques and methods

A brief introduction to the analytical ultracentrifugation of proteins for beginnersDiffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures -- Models for direct boundary fitting of indefinite ligand-linked self-association -...

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Körperschaft: Pembroke College (BerichterstatterIn)
Weitere Verfasser: Scott, David J. (HerausgeberIn), Harding, Stephen E. (BerichterstatterIn), Rowe, Arthur J. (BerichterstatterIn)
Format: UnknownFormat
Sprache:eng
Veröffentlicht: Cambridge Royal Society of Chemistry, RSC 2005
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Beschreibung
Zusammenfassung:A brief introduction to the analytical ultracentrifugation of proteins for beginnersDiffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures -- Models for direct boundary fitting of indefinite ligand-linked self-association -- Allowance for thermodynamic nonideality in sedimentation equilibrium -- Using analytical ultracentrifugation of DNA in CsCl gradients to explore large-scale properties of genomes -- LAMMNUM : a program to study self-associating macromolecules in sedimentation velocity experiments -- Correcting for the buoyancy of macromolecules : density increments and apparent partial specific volumes with particular reference to the study of membrane proteins -- Application of weighted robust regression to equilibrium ultracentrifugation -- The synthetic boundary technique of analytical ultracentrifugation : a valuable tool to study structure formation processes online -- Protein-DNA interactions studied at sedimentation equilibrium -- UltraScan : a comprehensive data analysis software package for analytical ultracentrifugation experiments -- Analysis of polysaccharide size, shape and interactions -- Analytical ultracentrifugation as a key complementary technique in structural biology -- Introduction to differential sedimentation -- Relating small angle scattering and analytical ultracentrifugation in multidomain proteins -- Modelling of biopolymers -- Fitting of thermodynamic data from equilibrium ultracentrifugation by robust least-squares with reduced parameter cross-correlation coefficients -- Calculation of volume, surface, and hydration properties of biopolymers -- Analysis of membrane proteins using analytical ultracentrifugation -- Solution properties of flexible macromolecules : theoretical and computational approaches -- The ELLIPS suite of whole-body protein conformation algorithms for Microsoft WINDOWS -- Weak interactions : optimal algorithms for their study in the AUC -- Analytical ultracentrifugation of colloids.
Beschreibung:Includes bibliographical references and index
Beschreibung:XXIII, 587 S.
Ill., graph. Darst.
24cm
ISBN:0854045473
0-85404-547-3