Chemical proteomics methods and protocols
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| Format: | UnknownFormat |
| Sprache: | eng |
| Veröffentlicht: |
New York u.a.
Humana Press
2012
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| Schriftenreihe: | Methods in molecular biology
803 |
| Schlagworte: | |
| Online Zugang: | Inhaltsverzeichnis |
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Tag hinzufügen | Beschreibung: | Includes bibliographical references and index Mass spectrometry-based chemoproteomic approaches -- Chemical proteomics in drug discovery -- Compound immobilization and drug-affinity chromatography -- Affinity-based chemoproteomics with small molecule-peptide conjugates -- A chemical proteomic probe for detecting dehydrogenases: Catechol rhodanine -- Probing proteomes with benzophenone photoprobes -- Biotinylated probes for the analysis of protein modification by electrophiles -- Profiling of methyltransferases and other S-adenosyl-l: -homocysteine-binding proteins by capture compound mass spectrometry -- Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC -- Determination of kinase inhibitor potencies in cell extracts by competition binding assays and isobaric mass tags -- Affinity-based profiling of dehydrogenase subproteomes -- Probing the specificity of protein-protein interactions by quantitative chemical proteomics -- Fluorescence-based proteasome activity profiling -- Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions -- Monitoring ligand modulation of protein-protein interactions by chemical cross-linking and high-mass MALDI mass spectrometry -- Time-controlled transcardiac perfusion crosslinking for in vivo interactome studies -- Ligand discovery using small-molecule microarrays -- Working with small molecules : preparing and storing stock solutions and determination of kinetic solubility -- A database for chemical proteomics : ChEBI -- Working with small molecules: Rules-of-thumb of Drug likeness |
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| Beschreibung: | XI, 313 S. Ill., graph. Darst. 27 cm |
| ISBN: | 9781617793639 978-1-61779-363-9 1617793639 1-61779-363-9 |