A comparison of some biological effects of three platinum (ii) compounds

Fayetteville, Univ. of Arkansas, Diss., 1979

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Bibliographische Detailangaben
1. Verfasser:	O'malley, James Dennis
Format:	UnknownFormat
Sprache:	eng
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Beschreibung
Zusammenfassung:	Fayetteville, Univ. of Arkansas, Diss., 1979 The three square-planar platinum II compounds--cis-dichlorodiammineplatinum II (cPDD), chloro(2,2',2''-terpyridine)platinum II (PTCl), and 2-hydroxyethanethiolato(2,2',2''-terpyridine)platinum II (PTHT)--bind to DNA bidentatively, monodentatively, and by intercalation respectively. All three complexes inhibit DNA synthesis in Escherichia coli by 70% to 80% with lesser effect on the synthesis of RNA and protein. The antitumor drug cPDD at 167 uM depresses the optical density (OD) of growing E. coli suspensions by 50%, causes elongation of the bacteria, induces the lambda prophage to lyse the lysogenic E. coli K12 host, and causes reversion of Salmonella typhimurium TA 98 and TA 100 strains in the Ames test; but has little effect with short treatment time on the infectivity of a T-series bacteriophage. PTCl and PTHT were originally synthesized to be used as nucleic acid dyes. PTCl at 150 uM depresses the OD of growing bacterial suspensions by 80%, but does not cause elongation of bacteria, does not induce the lambda prophage to lysis, and is not active in the Ames test. PTCl does interfere with the infectivity of a T-series phage treated outside of the host cell, and also stimulates RNA synthesis. PTHT at 271 uM does not affect the OD of growing bacterial suspensions, does not cause elongation of bacteria, does not induce lambda prophage to lysis, and is only weakly active in the Ames test with the TA 98 strain. PTHT will inhibit the infestivity of a T-series phage treated outside the host cell for a short time.
Beschreibung:	87 S.