˜Aœ comparison of the ultrastructure of compatible and incompatible interactions of xanthomonas campestris pv. malvacearum in cotton lines

Stillwater, Okla., Oklahoma State Univ., Diss., 1983

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Bibliographische Detailangaben
1. Verfasser: Al-mousawi, Ali Hashim
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Sprache:eng
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Zusammenfassung:Stillwater, Okla., Oklahoma State Univ., Diss., 1983
Scope and Method of Study. This investigation was conducted to compare the responses of two cotton lines to bacterial blight of cotton. Ac 44 a susceptible cotton line, was compared with the immune line Im 216, which is resistant to this bacteria. Plants of these lines were grown in a greenhouse. When cotyledons and foliage leaves were fully expanded, inoculum of 10('6) bacteria/ml was inoculated into Ac 44, while for Im 216 line a lower inoculum of 5 x 10('5) bacteria/ml was used. Samples for electron microscopy were taken at 0 time and every 12 hrs. after inoculation for 6 days from Ac 44, and at one day intervals from Im 216 for 6 days. Findings and Conclusion. Results of the ultrastructural study revealed changes in membrane systems of different cell organelles which started two days after inoculation in Ac 44. Three to 4 days post inoculation degeneration of mitochondrial membranes began following disappearance of chloroplast granal and stromal membranes. Nuclei appeared denser than normal, and coagulated ribosomes were seen after 5 days. These changes were followed by breaks in plant cell walls, through which bacteria entered the damaged cells. Fibrillar material was seen inside and outside cells at late stages of the disease, when water soaking symptoms appeared. Im 216 showed rapid desiccation of a few cells during the second day after inoculation. No hypersensitive response was macroscopically visible. The desiccated cells became disorganized and no organelles could be identified. The remaining cells appeared to be normal for the entire period of investigation. Bacteria were enveloped in fibrillar material at collapsed cell walls.
Beschreibung:43 S.