Mouse hematology a laboratory manual
Collection of peripheral blood -- Counting red blood cells, platelets, and viable nucleated white blood cells -- Protocol 1 determination of percent red cell volume (hematocrit) -- Protocol 2 methodology to determine a red blood cell count using a hemacytometer -- Protocol 3 methodology to determine...
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Format: | UnknownFormat |
Sprache: | eng |
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Cold Spring Harbor, N.Y.
Cold Spring Harbor Laboratory Press
c2010
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Zusammenfassung: | Collection of peripheral blood -- Counting red blood cells, platelets, and viable nucleated white blood cells -- Protocol 1 determination of percent red cell volume (hematocrit) -- Protocol 2 methodology to determine a red blood cell count using a hemacytometer -- Protocol 3 methodology to determine a platelet cell count using a unopette and a -- Hemacytometer -- Protocol 4 methodology to determine a white blood cell count and viability using a -- Hemacytometer -- Peripheral blood films and cytospin preparations -- Protocol 5 washing slides in preparation for microscopic examination of peripheral -- Blood and bone marrow -- Protocol 6 preparation of a peripheral blood film -- Protocol 7 fixing, staining, and mounting a peripheral blood film -- Protocol 8 preparation of a peripheral blood suspension for cytospin analysis -- Cell differential assessments of peripheral blood films -- Preparation of bone marrow for microscopic examination -- Protocol 9 bone marrow biopsy of the mouse -- Protocol 10 preparation of a bone marrow brush smear -- Protocol 11 preparation of a bone marrow suspension for cytospin analysis -- Cell differential assessments of bone marrow. |
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Beschreibung: | Includes bibliographical references and index. - Collection of peripheral blood -- Counting red blood cells, platelets, and viable nucleated white blood cells -- Protocol 1 determination of percent red cell volume (hematocrit) -- Protocol 2 methodology to determine a red blood cell count using a hemacytometer -- Protocol 3 methodology to determine a platelet cell count using a unopette and a -- Hemacytometer -- Protocol 4 methodology to determine a white blood cell count and viability using a -- Hemacytometer -- Peripheral blood films and cytospin preparations -- Protocol 5 washing slides in preparation for microscopic examination of peripheral -- Blood and bone marrow -- Protocol 6 preparation of a peripheral blood film -- Protocol 7 fixing, staining, and mounting a peripheral blood film -- Protocol 8 preparation of a peripheral blood suspension for cytospin analysis -- Cell differential assessments of peripheral blood films -- Preparation of bone marrow for microscopic examination -- Protocol 9 bone marrow biopsy of the mouse -- Protocol 10 preparation of a bone marrow brush smear -- Protocol 11 preparation of a bone marrow suspension for cytospin analysis -- Cell differential assessments of bone marrow |
Beschreibung: | XI, 99 S. Ill., graph. Darst. 26 cm 1 DVD-Video |
ISBN: | 9780879698850 978-0-87969-885-0 9780879698867 978-0-87969-886-7 |