Cell-free protein production methods and protocols
1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods / Takanori Kigawa -- 2. PURE Technology / Yoshihiro Shimizu and Takuya Ueda -- 3. The Cell-Free Protein Synthesis System from Wheat Germ / Kazuyuki Takai and Yaeta Endo -- 4. A Cell-Free Protein Synthesis System f...
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| Format: | UnknownFormat |
| Sprache: | eng |
| Veröffentlicht: |
New York, NY, Heidelberg u.a.
Humana Press
2010
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| Schriftenreihe: | Methods in molecular biology
607 |
| Schlagworte: | |
| Online Zugang: | Inhaltsverzeichnis |
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Tag hinzufügen | Zusammenfassung: | 1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods / Takanori Kigawa -- 2. PURE Technology / Yoshihiro Shimizu and Takuya Ueda -- 3. The Cell-Free Protein Synthesis System from Wheat Germ / Kazuyuki Takai and Yaeta Endo -- 4. A Cell-Free Protein Synthesis System from Insect Cells / Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, and Eiji Ando -- 5. Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells / Satoshi Mikami, Tominari Kobayashi, and Hiroaki Imataka -- 6. Analysis of Protein Functions through a Bacterial Cell-Free Protein Expression System / Takanori Kigawa -- 7. Cell-Free Based Protein Microarray Technology Using Agarose/DNA Microplate / Tatsuya Sawasaki and Yaeta Endo -- 8. An Efficient Approach to the Production of Vaccines Against the Malaria Parasite / Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, and Yaeta Endo -- 9. Protein Engineering Accelerated by Cell-Free Technology / Takuya Kanno and Yuzuru Tozawa -- 10. Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds / Takanori Kigawa -- 11. NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System / Toshiyuki Kohno -- 12. Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination / Shin-ichi Makino, Michael A. Goren, Brian G. Fox, and John L. Markley -- 13. Cell-Free Protein Synthesis for Structure Determination by X-Ray Crystallography / Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, and Ichizo Kobayashi -- 14. Production of Multi-Subunit Complexes on Liposome through an E. coli Cell-Free Expression System / Yutetsu Kuruma, Toshiharu Suzuki, and Takuya Ueda -- 15. Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System / Hiroyuki Hori -- 16. Strategies for the Cell-Free Expression of Membrane Proteins / Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch, and Frank Bernhard -- 17. Production of Membrane Proteins through the Wheat-Germ Cell-Free Technology / Akira Nozawa, Hideaki Nanamiya, and Yuzuru Tozawa -- 18. Ribosome Display with the PURE Technology / Takashi Kanamori, Hiroyuki Ohashi, and Takuya Ueda -- 19. Incorporation of 3-azidotyrosine into Proteins through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification / Takashi Yokogawa, Satoshi Ohno, and Kazuya Nishikawa -- 20. Synthesis of Functional Proteins within Liposomes / Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo |
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| Beschreibung: | XI, 261 S. Ill., graf. Darst. 26 cm |
| ISBN: | 9781603273305 978-1-60327-330-5 1603273301 1-60327-330-1 |