The condensed protocols from molecular cloning: a laboratory manual
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and ana...
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| Format: | UnknownFormat |
| Sprache: | eng |
| Veröffentlicht: |
Cold Spring Harbor, N.Y.
Cold Spring Harbor Laboratory Press
c2006
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| Online Zugang: | Table of contents |
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Tag hinzufügen | Zusammenfassung: | Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies. |
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| Beschreibung: | Includes index |
| Beschreibung: | V, 800 S. Ill., graph. Darst. |
| ISBN: | 9780879697723 978-0-87969-772-3 0879697725 0-87969-772-5 0879697717 0-87969-771-7 |