Pestivirus-Replikons als Werkzeug für heterologe Genexpression und molekulare Charakterisierung

Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2021

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Bibliographische Detailangaben
1. Verfasser: Schumann, Anja (VerfasserIn)
Körperschaft: Universität Greifswald (Grad-verleihende Institution)
Weitere Verfasser: Mettenleiter, Thomas C. (AkademischeR BetreuerIn), Tautz, Norbert (AkademischeR BetreuerIn)
Format: UnknownFormat
Sprache:ger
Veröffentlicht: Greifswald November 2020
Schlagworte:
Hochschulschrift > Pestvirus > Genexpression > Replikon
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Zusammenfassung:Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2021
Genexpression, Pestivirus, Replikon
Replicons are autonomously replicating RNA molecules incapable of generating infectious virus particles. They are important tools for virus characterization, expression of heterologous genes and for the development of vaccines. In the present study, a new replicon-based test system was established for the detection of antibodies specific for the atypical porcine Pestivirus (APPV). Based on a bovine viral diarrhea virus 1 (BVDV) clone, the original BVDV glycoproteins E1 and E2 were exchanged for the glycoproteins of APPV. Expression via replicons ensures the natural conformation of the proteins and thus the binding of the corresponding antibodies. This system allowed the serological analysis of 1115 pig sera without prior isolation or cell culture adaptation of the virus. With this newly developed system a seroprevalence study could be started and a high prevalence of APPV was demonstrated in the investigated German pig population. In the further course of this work replicons were used to characterize the atypical pestivirus Bungowannah Pestivirus (BuPV). While replicons with deletions of individual structural proteins C, E1 and E2 or of the entire structural protein region did not show any peculiarities compared to already investigated pestivirus replicons and could not produce infectious virions, BuPV replicons with ERNS deletion (BuPVΔERNS) showed the ability to undergo two further replication cycles and to infect cells, but with a very distinct growth deficit. The ERNS ...
Beschreibung:Literaturverzeichnis: Seite 84-96
Beschreibung:105 Seiten
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